Bacterial Colonization and Proliferation in Furcal Perforations Repaired by Different Materials: A Confocal Laser Scanning Microscopy Study

Following furcal perforation, bacteria may colonize the defect and cause inflammation periodontal destruction. This study used confocal laser scanning microscopy (CLSM) to evaluate Enterococcus faecalis colonization proliferation in perforations repaired with different materials. Furcal created 55 extracted human mandibular molars were using either MTA-Angelus, Endocem, or Biodentine coronally subjected E. suspension for 21 days. The specimens then stained a LIVE/DEAD Viability Kit visualized by CLSM. minimum maximum depths of bacterial penetration into dentinal tubules 159 1790 μM, respectively, mean 713 μM. There significantly more live than dead inside (p = 0.0023) all groups, three repair materials exhibited similarly sized area 0.083). However, there significant differences numbers at circumference perforation 0.0041), higher ratio MTA-Angelus group 0.001). repair, interface between material dentin penetrate through tubules. type has effect on viability colonizing bacteria.